t stat5 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc t stat5
T Stat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+stat5/Phospho-Stat5+(Tyr694)+Rabbit+mAb/bio_rxiv__64898__2026__01__23__701155-265-44-45
Average 96 stars, based on 461 article reviews

t stat5 - by Bioz Stars, 2026-10

96/100 stars

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Ubiquitin Proteomics:

Article Title: OSTM1 is a ubiquitin E3 ligase that suppresses B-cell malignancy by activating the cAMP/PKA/CREB pathway
Article Snippet: Cell lines were periodically authenticated by Short Tandem Repeat (STR) profiling at IDEXX BioResearch. .. Flag-M2 (Millipore Sigma, F1804), Flag (Millipore Sigma, F7425), 6X-His (Thermo Fisher Scientific, MA1-21315), p-AKT Ser-473 (Cell Signaling Technology, 4060), t-AKT (Cell Signaling Technology, 9272), GAPDH (ProteinTech, 10494-1-AP), p-S6 Ser235/236 (Cell Signaling Technology, 4858), t-S6 (Cell Signaling Technology, 2217), p-STAT5 Y694 (Cell Signaling Technology, 9359), t-STAT5 (Cell Signaling Technology, 25656), p-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (Cell Signaling Technology, 4376), t-p44/42 MAPK (Erk1/2) (Cell Signaling Technology, 9102), PDE3B (SMCP3B) (used for IB) (Novus Biologicals, NBP1-43333), PDE3B (used for IP) (Abcam, ab99290), Ubiquitin (P37) (Cell Signaling Technology, 58395), LC3B (Cell Signaling Technology, 2775), K48-linkage specific polyubiquitin (D9D5) (Cell Signaling Technology, 8081), GFP (Santa Cruz, sc-8334), BiP/GRP78 (BD Biosciences, 610978), calnexin (Transduction Labs, C45520-050), CLC7 (Thermo Fisher, A305-381A-T), p-CREB Ser133 (87G3) (Cell Signaling Technology, 9198), t-CREB (48H2) (Cell Signaling Technology, 9197), p-(Ser/Thr) PKA substrates (Cell Signaling Technology, 9621), OSTM1 (Millipore Sigma, ABN1365), goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 680 (Thermo Fisher, A-21057), goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa FluorTM 594 (Thermo Fisher, A11012), goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher, A11008), goat anti-rabbit secondary antibody (LI-COR, 926-32211), goat anti-rat secondary antibody for 680 channel (Thermo Fisher, A-21096) .. 6-thioguanine (6-TG) (MedchemExpress, HY-13765), biotin (Millipore Sigma, PHR1233), brefeldin A (Thermo Fisher, 00-4506-51), chloroquine (Selleckchem, S6999), concanavilin A (Vector Labs, B1005-5), cycloheximide (Millipore Sigma, C4859), L-glutamine (Fisher Scientific, 25030081), IRDye 800CW streptavidin (LI-COR Bio, 926-32230), IL-3 (mouse recombinant, carrier-free.

Transduction:

Incubation:

Article Title: Association between methylation of tumor suppressor gene SOCS1 and acute myeloid leukemia.
Article Snippet: Total proteins (50 μg) per sample were isolated by 10% SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore Corp, Billerica MA, USA). .. The membranes were probed with antibodies for SOCS1 (1:1,000; cat. no. 3950), t-JAK2 (1:1,000; cat. no. 4040), p-JAK2 (1:1,000; cat. no. 4406), t-STAT3 (1:1,000; cat. no. 9139), p-STAT3 (1:1,000; cat. no. 52075), t-STAT5 (1:1,000; cat. no. 25656), p-STAT5 (1:1,000; cat. no. 4322) and β-actin (1:2,000; cat no. 3700), and then incubated with anti-rabbit IgG, horseradish peroxidase (HRP)-linked secondary antibody (1:3,000; cat. no. 3700) (all from Cell Signaling Technology, Inc., Danvers, MA, USA) for 1 h for chemiluminescent detection. .. Specific bindings were visualized with Azure c500 (Azure Biosystems, Dublin, CA, USA).

Article Title: NAMPT mediates PDGF-induced pulmonary arterial smooth muscle cell proliferation by TLR4/NF-κB/PLK4 signaling pathway.
Article Snippet: Nicotinamide phosphoribosyltransferase (NAMPT), a pleiotropic protein, promotes the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs), which is associated with the genesis and progression of pulmonary arterial hypertension (PAH).. NAMPT is highly increased in PAH patient’s plasma and highly relevant to PAH severity.. The mRNA and protein levels of NAMPT are elevated in PAH animal models.

Article Title: DNMTs-mediated SOCS3 methylation promotes the occurrence and development of AML.
Article Snippet: Yunguo Zhao, Department of Medicine, Handan Central Hospital, No. 15 Zhonghua South Street, Handan, 056001, Hebei, China.. Email: zyg1966@163.com Abstract Objectives: As a tumor suppressor gene, SOCS3 inhibits the growth of tumor cells by regulating JAK/STAT signaling pathway through negative feedback.. This study aimed to investigate the biological function and mechanism of SOCS3 methylation mediated by DNMTs in the development of AML.

Phospho-proteomics:

Article Title: Growth Hormone Upregulates Mediators of Melanoma Drug Efflux and Epithelial-to-Mesenchymal Transition In Vitro and In Vivo
Article Snippet: A universal scrambled siRNA duplex (Origene #SR30004) was used as control. .. Protein extracted from B16-F10 and SK-MEL-30 cells treated with or without GH, and tumors was tested for the phosphorylation of STAT5, ERK1/2, AKT, SRC, CDH1, CDH2, ZEB1, SNAI2, SNAIL/SNAI1, VIM, b-CAT, t-ERK1/2, t-AKT, ABCC1, ABCB1, ABCG2, IGF1R beta, p-STAT1, t-STAT1, p-STAT3, t-STAT3, t-STAT5 (CST #9351, #4370, #4058, #2101, #3195, #13116, #3396, #9585, #3879, #2101, #5741, #8480, #9102, #4685, #72202, #13342, #42078, #3027, #9167, #14994, #9145, #12640, #94205), and ABCG1 (NB400-132, Novus Biologicals, Littleton, CO, USA) to determine their activation or expression induced by GH as previously described [ ]. β-Actin, GAPDH (#4970, #5174, CST, Danvers, MA, USA) or total protein staining (#926-11011, LI-COR, Lincoln, NE, USA) were used as loading controls. .. Densitometry analysis of individual blots was performed using Image Studio LITE Ver 5.2 (LI-COR, Lincoln, NE, USA).

Activation Assay:

Expressing:

Staining:

Software:

Western Blot:

Article Title: GALNT3 is a novel target driving lymphomagenesis via O-glycosylation of FGFR2
Article Snippet: All compounds, except for in vivo studies, were reconstituted in the DMSO (Abcone, Cat No. D26563 ), stored at 100 mM stock concentrations in -80 °C, and used at the indicated doses as suggested by the vendor. .. Immunoblotting antibodies CDK2 (Cat No. 2546T), CDT1 (Cat No. 8064T), cyclin A2 (Cat No. 91500T), cyclin E1 (Cat No. 20808T), cyclin D1 (Cat No. 2978T), Geminin (Cat No. 52508T), p-ERK (Cat No. 4370T), t-ERK (Cat No.4692T), p-AKT (Cat No.4060T), t-AKT (Cat No.4691 S), p-STAT5 (Cat No.4322T), t-STAT5 (Cat No. 9420 S) were purchased from Cell Signaling Technology (Danvers, USA); GALNT3 (Cat No.4314 S) was supplied by GeneTex Inc. FGFR2 (Cat No. AB289968 ) was purchased from Abcam (Waltham, USA). b-tubulin (Cat No. 12987-1-AP) and GAPDH (Cat No. 60008-1-Ig) antibodies were purchased from Proteintech (Rosemont, USA). .. Cell proliferation was analyzed using a Cell Counting Kit-8 (YEASEN, China).

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